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. 2016 Mar 30;64(4):256–267. doi: 10.1369/0022155416639013

Figure 4.

Figure 4.

Summary of the results in paired primary and metastatic breast cancer samples. The heatmap displays the results of ER, PgR, HER2 and Ki67 IHC investigated by repeated immunoprofiling in paired primary and metastatic breast cancer samples. The panel shows all 56 cases displayed with both IHC rounds, their resulting subtypes, the discrepancies of rounds 1 and 2, the consensus subtypes in both primaries and metastases, and the alteration of immunoprofile between the primary tumors and the distant metastases. For rounds 1 and 2 and consensus lines: bright green, ER score <3, PgR score <3, HER2 negative, Ki67 index ≤14%; red, ER score ≥3, PgR score ≥3, HER2 positive/amplified, Ki67 index >14%; light blue, luminal A; light green, luminal B (all); pink, HER2+; light yellow, TNBC. The “Discrepancy” lines show whether rounds 1 and 2 resulted in similar results. The “Alteration” line shows change in subtype in metastasis: red, no change due to consistency; green, inconsistent results; gray, no subtype assigned in any of the rounds. Consensus subtype is established with a combination of assignments based on both rounds in primaries and metastases alike. The line location shows where the actual sample is located: orange, bone (B); brown, CNS (C); purple, lung (L).