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. 2015 Apr 21;23(6):1077–1091. doi: 10.1038/mt.2015.46

Figure 1.

Figure 1

Differentiation of neural stem/progenitor cells (NS/PCs) following stimulation with M0, M1- or M2-CM in vitro. (a,b) Representative immunofluorescence images of NS/PCs differentiated into Tuj1-, Oligo2-, and glial fibrillary acidic protein (GFAP)-positive (+) cells without (control, CTL) or with the assorted CM samples. The enlarged cells in the lower left-hand corner of each panel show the typical morphology for each culture. Space bars = 200 μm. (c–e) Percentage of Tuj1-, Oligo2-, and GFAP-positive cells differentiated from NS/PCs without (control) or with the assorted CM samples. (f–h) qPCR analysis of tuj1, pdgfra, and gfap, (i) western blotting of Tuj1, Oligo2, and GFAP (j–l) a densitometric quantitative analysis in cells differentiated from NS/PCs without (CTL) or with the assorted CM samples. Data in (c–h) and (j–l) were pooled from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001); data in (a, b, and i) are representative of three independent experiments.