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. 2015 Apr 21;23(6):1077–1091. doi: 10.1038/mt.2015.46

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Differentiation of engrafted neural stem/progenitor cells (NS/PCs) cotransplanted with M0, M1, and M2 macrophages into the intact spinal cord. (a–c) Representative images of NS/PCs derived from GFP-Tg mice and cotransplanted with M0, M1, or M2 macrophages. The NS/PCs were differentiated into (a) NeuN-, (b) MBP-, and (c) glial fibrillary acidic protein (GFAP)-positive cells and detected by IHC. The boxed area in each image is enlarged at the lower left-hand corner of the panel. Space bars = 50 μm. (d–f) The percentages of (d) NeuN-, (e) MBP-, and (f) GFAP-positive cells among the engrafted GFP-expressing NS/PCs are shown. Data in (a–c) are representative of three independent experiments; data in (d–f) were pooled from three independent experiments (*P < 0.05, **P < 0.01).