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. 2015 Apr 21;23(6):1077–1091. doi: 10.1038/mt.2015.46

Figure 4.

Figure 4

Differentiation of engrafted neural stem/progenitor cells (NS/PCs) cotransplanted with polarized macrophages into the acutely injured spinal cord. (a–c) Representative images of engrafted NS/PCs derived from GFP-Tg mice and cotransplanted with M0, M1, or M2 macrophages. The NS/PCs were differentiated into (a) NeuN-, (b) MBP-, and (c) glial fibrillary acidic protein (GFAP)-positive cells and detected by IHC. The boxed area in each image is enlarged at the lower left-hand corner of the panel. Space bar = 50 μm. (d–f) The percentages of (d) NeuN-, (e) MBP-, and (f) GFAP-positive cells among the engrafted GFP-positive NS/PCs are shown. Data in (a–c) are representative of three independent experiments; data in (d–f) were pooled from three independent experiments (*P < 0.05, **P < 0.01). (g) BMS scores were evaluated in SCI mice engrafted with NS/PCs and M0, M1, or M2 macrophages (n = 6 mice per group). M2 + NS/PC group versus M1 + NS/PC group, *P < 0.05, **P < 0.01; M2 + NS/PC group versus M0 + NS/PC group, #P < 0.05. (h) The expression of synapsin is shown in the injured spinal cord in the three experimental groups. Space bar = 20 μm.