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. 2015 Apr 21;23(6):1077–1091. doi: 10.1038/mt.2015.46

Figure 5.

Figure 5

Migratory patterns of engrafted neural stem/progenitor cell (NS/PC)-derived cells in the injured spinal cord after co-transplantation of NS/PCs and M0, M1, or M2 macrophages. (a–d) Migration of NS/PCs alone without cotransplanted macrophages (a) and with cotransplanted (b) M0, (c) M1, and (d) M2 macrophages. The boxed areas in (a–d) are enlarged in (a'–d'). In a–d, ** denotes the lesion epicenter. Space bars = 1 mm. (a') shows the middle region of the rostral migration stream of the NS/PC-derived cells, while (b'–d') all show end-regions of the rostral migration stream. The arrowheads in (a'–d') indicate GFP-positive NS/PC-derived cells. Space bar = 50 μm. (e) Statistical results showing the integrated optical density (IOD) and the migration distance (P1, P2, P3, and P4) of engrafted NS/PC-derived cells cotransplanted without or with polarized macrophages in injured spinal cord. M1+NS/PCs versus M2+NS/PCs, P1 < 0.001; NS/PCs versus M2+NS/PCs, P2 < 0.001; M0+NS/PCs versus M2+NS/PCs, P3 < 0.001; M0+NS/PCs versus M1+NS/PCs, P4 < 0.01. Statistical data came from three independent experiments. D, dorsal; R, rostral.