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. 2015 Apr 21;23(6):1077–1091. doi: 10.1038/mt.2015.46

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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CXCR4/CXCL12 signaling contributes to the migration of neural stem/progenitor cell (NS/PC)-derived cells in response to polarized macrophages. (a–d) The mRNA expression levels of cxcr4 (a and b) and cxcl12 (c and d) are shown in (a and c) M0-, M1-, and M2-CM-treated NS/PCs and (b and d) M0, M1, and M2 macrophages in vitro. (e) Western blot analysis of CXCR4 expression in M0-, M1-, and M2-CM-treated NS/PCs. Spleen tissue was used as the positive control. (f) Quantitative densitometric analysis indicated that M1-CM-treated NS/PCs expressed elevated levels of CXCR4. (g) CXCL12 expression in NS/PCs cultured with M0, M1-, or M2-CM, as well as in M0, M1, and M2 macrophages. Space bar = 10 μm. (h) IHC images of CXCL12 expression in engrafted NS/PC-derived cells cotransplanted with macrophages into the injured spinal cord. Space bar = 10 μm. (i) NS/PC migration induced by M0, M1-, or M2-CM in a transwell assay with and without AMD3100. Space bar = 50 μm. Data in (a–d) and (f) were pooled from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001).