Figure 5.

Left ventricular cNIS expression after endocardial injection. (a) Diagram of the left ventricular myocardial sampling. Samples are numbered and the positions of each segment analyzed are written in italic using the following abbreviations: ant, anterior; lat, lateral; post, posterior; inf, inferior; sept, septum. The main arteries are also presented in red: LAD, left anterior descending (artery); LCX, left circumflex (coronary artery); Cx, circumflex; RCA, right coronary artery. (b) Western blots of cNIS in the 16 LV segments sampled from dogs 11 to 13 administered AAV9-cNIS endocardialy. The bands displayed correspond to the specific semiglycosylated form ~70 KDa (see Supplementary Figure S2). Absolute detection of NIS protein by IHC is reported in the corresponding segments below each Western blot (any⁺ or none^–). (c) Full thickness LV sample from segment 10 of dog 13, which is a representative of NIS positive samples from the endocardial administration group (dogs 11–13). Bar = 3 mm. Panels (d–f) are respective magnifications of the endocardium, midmyocardium, and subepicardium areas boxed on picture c. Bar = 200 μm. AAV, adeno-associated virus; cNIS, canine sodium iodide symporter; IHC, immunohistochemistry.