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. 2015 May 19;23(7):1189–1200. doi: 10.1038/mt.2015.66

Figure 2.

Figure 2

The surge in monocyte/macrophage IGF-1 production correlates with transition from inflammatory to repair macrophage phenotypes following muscle injury. (a) Kinetics of Ly6C+ monocyte/macrophage and CD206+ macrophage accumulation in the quadricep muscle is presented at various time points after CTX injury in WT muscle. (b) For analysis of monocyte/macrophage subpopulations, mononuclear cells were isolated from quadricep muscles and analyzed by flow cytometry. Monocytes/macrophages were defined as CD45+CD11b+F4/80+ as shown in the first two images, and then, the inflammatory monocyte/macrophages were discriminated by Ly6C expression while a mature reparative macrophage population was defined by CD206 expression. Isotype controls were used as negative gating controls to define positive signals and are shown in Supplementary Figure S2. (c) IGF-1Ea and IGF-1Eb propeptide expression was determined by qPCR of whole muscle (black bars) as well as Ly6C+ (red bars) and CD206+ (blue bars) macrophages isolated from CTX-injured muscle at 0, 2, 5, and 10 days after CTX injury. (d) MCSF expression measured by qPCR in whole muscle at 0, 2, 5, and 10 days after CTX injury. n = 7–8. Data represent mean ± SEM. CTX, cardiotoxin; WT, wild type.