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. 2015 Nov 17;24(2):331–341. doi: 10.1038/mt.2015.195

Figure 3.

Figure 3

Glucocorticoid (GC)-priming effect on duration of enhanced transfection outcomes. (a) The increase in transgene expression due to GC-priming remains statistically increased over unprimed cells for at least 72 hours. At 72 hours, all donors express the luciferase transgene at near zero relative light unit/mg total protein (RLU/mg), while GC-primed cells not only express transgene at appreciable levels, but donors 1–3 express transgene at levels greater than the highest levels without GCs (between 72 hours DEX versus 24 hours BM and EtOH, donors 1 and 2, P ≤ 0.001; D3, P ≤ 0.01). Error bars represent standard error (SE) of the mean for triplicate wells collected on duplicate days. (b) In addition to prolonged transgene expression levels, the increase in transfection efficiency is also maintained for at least 72 hours when compared to unprimed cells. Error bars represent SE of the mean for pooled triplicate wells collected on triplicate days.