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Analysis of U373 cells isolated from recurrent tumors. U373 cells were isolated from recurrent tumor of mice that were treated with IL13Rα2-CAR T cells. After short-term culture (2–7 days), FACS analysis and cytotoxicity assays were performed. (a) FACS analysis for IL13Rα2. (b) IL13Rα2-CAR T cells killed U373 tumor cells isolated from recurrent tumors in contrast to Raji cells in a standard 4-hour cytotoxicity assay. CAR, chimeric antigen receptor.
