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. 2016 Jan 19;24(2):385–397. doi: 10.1038/mt.2015.215

Figure 3.

Figure 3

Lipid 1 is the most efficient among the lipids 1–3 in transfecting dendritic cells (DCs) in vivo. (a) In vivo DC transfection properties of the lipoplexes of lipids 1–3. Lipoplexes of lipids 1–3 and α5-GFP plasmids were injected (s.c.) into C57BL/6J mice and draining lymph nodes were harvested after 24 hours. Cells were labeled with anti-CD11c magnetic beads and enriched in an Midimax columns and the populations of GFP-positive cells were analyzed by flow cytometry. Cells collected from lymph nodes of mice injected (s.c.) with only vehicle (5% aqueous glucose) were used as negative control. Each experiment was repeated three times and similar FACS profiles were observed in each time. (b) Graphical representation of GFP-positive CD11C+ cells isolated from lymph nodes (*P < 0.05 versus lipoplex of lipid 3 and α5-GFP).