Figure 6.

Antitumor efficacies of the lipoplex of lipid 1 and p-CMV-MART1 under therapeutic settings. (a) Six- to eight-week-old female C57BL/6J mice (each weighing 20–22 g) were injected with ~1 × 10⁵ B16F10 cells s.c. in the right flank. On day 15, mice were randomly sorted into four groups (n = 5) and each group was immunized s.c. with one of the following: lipoplexes of lipid 1 and p-CMV-MART1 (square); lipid 2 and p-CMV-MART1 (triangle); lipid 3 and p-CMV-MART1 (circle). One group was injected with 5% glucose (vehicle control, diamond). This treatment was repeated on days 18 and 21 after tumor cell implantation. Tumor volumes (V = ½ ab² where, a = maximum length of the tumor and b = minimum length of the tumor measured perpendicular to each other) were measured with a slide caliper for up to 25 days (*P < 0.05 versus lipid 1/p-CMV-MART1). (b) Survival study.