Figure 1.

rAAV integrations in Rian and Mir341 after gene therapy. rAAV integrations from published data sets,^2,4,11 designated in the figure by first author, were extracted and filtered for those that mapped into (a) Rian and (b) Mir341 and then uploaded to the UCSC Genome Browser (Genome Reference Consortium Mouse Build 38) to display the exact location of each integration.^18,19 A MULTIZ alignment of 60 vertebrate genomes centered on the murine Mir341 locus depicts only six vertebrate sequences, because no homology was detected for other vertebrates. The locations within Mir341 of two simple repeats—CTCG (pink) and CGGT (orange)—as well as CpGs (blue) are displayed. Chandler and colleagues' gray AAV integrations mapped into Mir341 but were not associated with HCC, as detailed in ref. 2.