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Osteoclast-like activity was identified using tartrate-specific acid phosphatase (TRAP) staining as large multinucleated cells at the bone perimeter within resorption lacuna (image A and B taken at 400×). TRAP analysis (C) shows a predominance of osteoclast-like cells in calvaria treated with the PBS treated group (A), and is decreased with local delivery of 7ND (B). Red arrow: osteoclast-like cells. ***p < 0.001.
