Figure 1. Unbiased detection of major planarian cell types by SCS.

(A) Illustration of SCS data generation and analysis. Animals were cut postpharyngeally (red line) and wound sites (red box) were isolated at 3 time points. Wound tissue was macerated, and dividing (4C) or non-dividing (2C) cells were isolated by FACS (Methods; dashed line shows gates). Sequencing libraries were prepared by cDNA-amplification and shearing, and libraries were sequenced and analyzed. (B) t-SNE plot of single cells. Cells (colored dots) are grouped by density clustering and labeled based on marker analysis. Cells shown are from the 2C (wounded and unwounded) and 4C (wounded) fractions. (C) Expression of canonical cell-type markers overlaid on t-SNE plots of the single-cells (dots); low and high ranked expression are colored by a gradient of blue, yellow, and red. (D) Analysis of the neoblast compartment. Shown are neoblasts (dots) from uninjured animals. Clusters are annotated based on multiple neoblast markers. (E) Expression of class-specific neoblast markers. See also Figure S1-2.