Figure 4. notum is the only gene detectably induced asymmetrically at wounds.

(A) The gene expression profiles of injuries with different wound orientation (anterior and posterior; left panel) are compared in time-course experiments of tissues isolated from the same location. Plotted is the log₂ ratio of differentially expressed genes between the two wound types (FDR ≤ 0.05; fold-change ≥ 1.5). Dashed lines represent genes that could not be validated by WISH, and that are likely false-positives. (B) WISH validations of wound-induced genes shown in panel A (performed on at least 10 animals). Top panel shows gene expression in intact animals compared to the expression in amputated trunks (bottom panel). Amputated animals were fixed at the time point showing peak asymmetry in expression. Only notum showed asymmetrical expression following wounding (black arrow). Scale=100 μm. See also Figure S3.