Figure 5.

S100A1 prevents premature diastolic Ca²⁺ waves during EC coupling in electro-mechanically coupled ventricular cardiomyocytes with “leaky” RyR2. (a) and (c) Representative tracings of Ca²⁺ transients of electrically-paced and to Ca²⁺ sensitized RyR2s basal and ISO-stimulated (100 nmol/l) control (AdGFP) and S100A1-treated (AdS100A1) NCs (left) and FCs (right). (b) and (d) Statistical analysis of diastolic Ca²⁺ waves and systolic Ca²⁺ transient amplitudes in electrically paced NCs (b) and FCs (d). Number of cells analyzed per group is shown on top of each bar. ^#P < 0.05 versus ISO. Data are given as mean ± SEM.