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. 2015 Jun 2;23(8):1380–1390. doi: 10.1038/mt.2015.71

Figure 2.

Figure 2

Efficiency of PD-1 gene disruption following electroporation with mRNA encoding PD-1-targeted zinc finger nucleases (ZFNs). Cel-1 surveyor nuclease assay performed 3 days following electroporation. ZFN-dependent gene editing occurred in 46.0% of alleles when tumor infiltrating lymphocytes from Donor 1 were electroporated using a smaller scale cuvette and 42.0% when electroporated using the flow electroporation processing assembly. Gene editing occurred in 37.0% of alleles in Donor 2 and 37.0% in Donor 3.