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. 2015 Jul 7;23(9):1486–1496. doi: 10.1038/mt.2015.108

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Copyright © 2015 American Society of Gene & Cell Therapy

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Circulating tumor cell (CTC) identification in clinical blood samples. (a) Blood sample preparation. Red blood cells were lysed with hypotonic NH₄Cl solution followed by incubation with hypotonic NaCl. (b) Costaining of CTCs from blood of a patient with lung adenocarcinoma by a Cy-5-labeled LC-18 aptamer and FITC-labeled anti-pan cytokeratin antibodies. (c) Costaining of CTCs from blood of a patient with squamous lung cancer by Cy-5-labeled LC-18 aptamer and FITC-labeled anti-pan cytokeratin antibodies. (d) Costaining of CTCs from blood of a colon cancer patient with lung metastases by Cy-5-labeled LC-18 aptamer and FITC-labeled anti-pan cytokeratin antibodies. Magnification ×60.