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. 2015 Aug 4;23(10):1653–1662. doi: 10.1038/mt.2015.120

Figure 3.

Figure 3

ISG15 induces polyfunctional HPV16 E7-specific CD8 T-cells. (a) Schematic diagram of gating strategy used to identify Ag-specific CD8 T-cell populations. (b–d) Column graphs show the percentages of HPV16 E7-specific CD8 T-cells releasing total cytokines IFNγ (b), TNFα (c), and IL-2 (d) after stimulation with DbE749-57-specific peptide. (e) Column chart show polyfunctional subpopulations of single-, double-, or triple-positive CD8 T-cells releasing effector cytokines: IFNγ, TNFα, and IL-2 to E749-57-specific stimulation. Pie charts represent proportion of each cytokine population. Experiments were performed at least two times with similar results with four to five mice per group. All cell counts are relative and not absolute. *P < 0.05 compared with HPV16 group. Error bars indicate standard error of the mean.