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. 2015 Jul 7;23(10):1663–1670. doi: 10.1038/mt.2015.107

Figure 2.

Figure 2

Sera from hICAM-1 Tg mice immunized with HRV-HIV chimera bind 4E10 peptide as well as HIV-1 SF162 gp140 trimer. (a) Serial dilutions of sera (week 9) were tested for binding 4E10 peptide (K7-D11 lactam-bridged Ac-NWFDITK7WLWD11KKK-NH2) by enzyme-linked immunosorbent assay (ELISA). Each serum sample was tested in triplicate and mean titers are shown for each mouse. The antibody titers were calculated by relating OD450 values of sera to that obtained with the ref 4E10 Mab and is shown as µg/ml. (b) Immunized mice sera (week 9) were tested for binding HIV-1 SF162 gp140 trimer at 1: 50 dilution by ELISA. Antibody titers were calculated as in (a). Unpaired Student t-test was used to calculate the significance between groups and P values are indicated.