Skip to main content
. 2015 Jul 7;23(10):1663–1670. doi: 10.1038/mt.2015.107

Figure 3.

Figure 3

Neutralizing ability of immunized mice sera against HIVBAL assessed by Tat-regulated luciferase assay and p24 enzyme-linked immunosorbent assay (ELISA). (a) HIV-BAL (200 pg of p24) was incubated with 1:50 dilution of sera for 2 hours at 37 °C before infecting TZM-bl cells and 24 hours after infection, the cell lysates were tested for luciferase activity. (b) HIV-BAL (200 pg of p24) was incubated with 1:10 dilution of sera for 2 hours at 37 °C before infecting TZM-bl cells and 48 hours after infection, the culture supernatants were tested for p24 antigen levels using p24 ELISA kit (from PerkinElmer). Preimmune sera and 12B1-immunized wild-type C57 mice were used as negative controls. Unpaired Student t-test was used to calculate the significance between preimmune sera group and sample groups. P values are indicated. Unpaired Student t-test was also used to compare between HRV14 immunized group and sample groups and the results were also significant, but have not been shown here.