Figure 4.

NKG2D-CAR-T cell toxicity is exacerbated by pre-conditioning with chemotherapy. Mice were treated with 150 mg/kg cyclophosphamide (CTX) intraperitoneally 24 hours prior to adoptive transfer. (a–c) BALB/c/c mice were treated with 10⁷ NK-CAR-T cells intravenously as indicated (n = 6). (a) Temperatures, (b) weight loss, and (c) survival were monitored over the course of 7 days post adoptive cell transfer (ACT). (d–f) Naive C57BL/6 mice were treated as in a (n = 5–10). (d) Temperatures are shown at the peak drop of 8 hours post ACT. (e) Weight loss and (f) survival were monitored over 7 days post-ACT. Temperature and weight loss data are presented as mean ± SEM. Dotted lines indicate data from surviving animals.