Figure 1.

Vectofusin-1 enhances CD34⁺ hematopoietic stem/progenitor cells transduction with various lentiviral or retroviral pseudotypes. (a) Schematic representation of the primary sequence of Vectofusin-1 peptide composed of four different types of residue: lysine (K), histidine (H), leucine (L), and alanine (A); carboxy-terminal amidation (-NH₂). (b–e) A variety of green fluorescent protein (GFP)-encoding vectors (GALVTR-LVs (n = 5), RD114TR-LVs (n = 3), MLV-A-LVs (n = 3), GALV-MLV (n = 3)) were used to transduce hCD34⁺ cells during 6 hours in the absence (none) or presence of Vectofusin-1 (12 µg/ml). Data are shown as the average percentage of GFP⁺ or 7-AAD⁺ cells ± SD from number of umbilical cord blood samples treated in duplicate (**P < 0.01; ***P < 0.001, Student's t-test). 7-AAD, 7-aminoactinomycin D; GALV, gibbon ape leukemia virus; LV, lentiviral vector; MLV-A, amphotropic murine leukemia virus; MOI, multiplicity of infection; TU, transducing unit.