Figure 3.

Comparison of Vectofusin-1 with other culture additives to promote transduction of hCD34⁺ cells with purified vesicular stomatitis virus-G-lentiviral vectors (VSV-G-LVs). hCD34⁺ cells (three umbilical cord blood donors tested in three independent experiments) were infected with increasing concentrations of purified VSV-G-LVs (10⁷, 5 × 10⁷, and 10⁸ ig/ml corresponding to multiplicity of infection (MOI) 80, 400, and 800, respectively) in the absence (none) or presence of indicated transduction enhancers. (a) Transduction was measured in the bulk of cultured cells after 5 days by following the percentage of GFP⁺ cells ± SD using flow cytometry (**P < 0.01; *P < 0.05, Student's t-test) and (b) average vector copy number (VCN) of the cell population by quantitative PCR. GFP, green fluorescent protein; ig, infectious genome; SEVI, semen enhancer of viral infection.