Figure 2.

Multipotent adult progenitor cells (MAPC) prevent HP-induced enhancement of effector T-cell cytokine responses. Untouched CD4+ T-cells were cocultured at a ratio of 1:5 with autologous monocytes in the presence and absence of IL-7 (50 ng/ml) with or without MAPC for 6 days. Cultures were then restimulated for 4 hours with 50 ng/ml PMA and 1 μg/ml Io. (a,d) Representative fluorescence-activated cell sorting (FACS) plots (MAPC at 1:2) and (b–f) bar graphs (ratios indicated) of data from ICS analysis of cocultures; gates were set on CD3+ viable cells. (g) Representative FACS plots and schematic of experimental design for sorted T-cell restimulation assays. CTV-labeled CD4 T-cells were cocultured with monocytes in the presence or absence of IL-7 as above with or without MAPC for 6 days at a low ratio of 1 MAPC: 40 CD4 T-cells. Under which conditions suppression of proliferation was minimal in the donors used. Untouched CD4 T-cells from all three conditions were FACS sorted and subsequently cocultured with allogeneic DC (1:8) for 72 hours. Harvested S/N were used for cytokine bead array analysis and the levels of (h) IFN-γ measured, *P < 0.05; NA, not activated; ns, not significant. Error bars represent the mean ± SEM of five donors. Data are representative of three independent experiments. CTV, cell trace violet; MSC, mesenchymal stromal cell.