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. 2015 Sep 1;23(11):1712–1721. doi: 10.1038/mt.2015.142

Figure 2.

Figure 2

CD74 suppresses Aβ production from neural progenitor cell (NPC)-derived cultured neurons. (a) Experimental designs for in vitro Aβ production assay. NPC-derived neurons (4 × 105 cells/well in 24-well plates) were infected with adenovirus expressing APPsw (AdAPPsw) or green fluorescent protein (GFP) (AdGFP), followed by transduction of AAV-TRE-GFP or CD74 (2 × 109 vg, cotransduced with AAV-tTA at 2 × 109 vg) for 3 days. Neuron culture media were subjected to Aβ40 or Aβ42 ELISA. (b,c) NPC-derived neurons were infected with adenovirus expressing GFP or APPsw (AdGFP or AdAPPsw), followed by transduction of AAV-tTA only or plus AAV-TRE-GFP or CD74. Aβ40 (b) or Aβ42 (c) production was quantified. Bars represent mean ± standard error of the mean. ** or *** denotes P < 0.01 or 0.001 versus AdAPPsw only, plus AAV-tTA or plus AAV-TRE-GFP as determined by one-way analysis of variance, Newman-Keuls post hoc test. AAV, adeno-associated virus; ELISA, enzyme-linked immunosorbent assay; TRE, tet-response element; tTA, tetracycline (tet)-controlled transactivator.