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Subcellular localization of amyloid-β precursor protein (APP) and Lamp1 in CD74-overexpressing neurons. Confocal microscopy shows cellular localization of Lamp1 lysosomal compartment (red), APP (green) and GFP or CD74 (pseudo-colored blue). Merged images of Lamp1 and APP with or without GFP/CD74 are shown. APP co-localization with Lamp1 (arrowheads) was quantified by ImageJ with a colocalization plugin (n = 10). Scale bar = 10 μm. GFP, green fluorescent protein.
