Table 1. ORF4 directly associates with multiple viral proteins: Yeast Two Hybrid analysis of ORF4 interaction partners in g-1 HEV.
| YEAST COTRANSFORMANTS | LT- | LTHA- | LTH-A+ | LT- + X-α-gal | LTH-+3AT(mM) | ||
|---|---|---|---|---|---|---|---|
| 5 | 10 | 20 | |||||
| Y2H GOLD | - | - | - | - | - | - | - |
| BD-ORF4+AD | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-ORF3 | +++ | +++ | +++ | +++ | + | - | - |
| BD-ORF4+AD-X | +++ | +++ | +++ | +++ | +++ | ++ | + |
| BD-ORF4+AD-Helicase | +++ | +++ | ++ | ++ | + | - | - |
| BD-ORF4+AD-Methyl transferase | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-Protease | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-Y domain | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-V domain | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-D domain | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-RdRp | +++ | - | - | - | - | - | - |
| BD-ORF4+AD-ORF2 | +++ | - | - | - | - | - | - |
| BD+AD-ORF3 | +++ | - | - | - | - | - | - |
| BD+AD-X | +++ | - | - | - | - | - | - |
| BD+AD-Helicase | +++ | - | - | - | - | - | - |
| BD-ORF3+AD-TSG101 | +++ | +++ | +++ | +++ | +++ | ++ | + |
| BD-ORF4+AD-g-3 ORF3 | +++ | + | + | - | - | - | - |
| BD-ORF4+AD-g-3 X | +++ | +++ | +++ | +++ | +++ | ++ | + |
| BD-ORF4+AD-g-3 Helicase | +++ | - | - | - | - | - | - |
LT-: Leucine, Tryptophan deficient medium; LTHA-: Leucine, Tryptophan, Histidine and Adenine Hemisulphate deficient medium; LTH-A+: Leucine, Tryptophan, Histidine deficient medium supplemented with Aureobasidin; LT- + X-α-gal: Leucine, Tryptophan deficient medium supplemented with X-α-gal; 3AT: 3-amino 1,2,4- Triazole. +++ Strong growth, ++ average growth, + poor growth,—no growth. BD: Binding domain, AD: Activation domain. Data represents the summary of 3 independent experiments.