Table 4. Yeast Two Hybrid screening of host factors that directly interact with g-3 HEV RdRp.
| YEAST COTRANSFORMANTS | LT- | LTHA- | LTH-A+ | LT-+X-α-gal | LTH-+3AT(mM) | PPI status | ||
|---|---|---|---|---|---|---|---|---|
| 5 | 10 | 20 | ||||||
| Y2H GOLD | - | - | - | - | - | - | - | |
| BD-g-3 RdRp+AD | +++ | + | ++ | - | + | - | - | |
| BD-g-3 RdRp+AD-COG1 | +++ | ++ | ++ | - | + | - | - | N |
| BD-g-3 RdRp+AD-RTN1 | +++ | + | +++ | + | + | - | - | N |
| BD-g-3 RdRp+AD-EEF1A1 | +++ | +++ | +++ | + | +++ | +++ | ++ | Y |
| BD-g-3 RdRp+AD-TUBB | +++ | +++ | +++ | + | +++ | +++ | +++ | Y |
| BD-g-3 RdRp+AD-ZDHHC6 | +++ | +++ | +++ | + | +++ | +++ | ++ | Y |
| BD-g-3 RdRp+AD-MACF1 | +++ | +++ | +++ | + | +++ | +++ | ++ | Y |
| BD-g-3 RdRp+AD-TMEM66 | +++ | ++ | +++ | + | ++ | + | - | Y |
| BD-g-3 RdRp+AD-ACTG1 | +++ | +++ | +++ | + | +++ | +++ | +++ | Y |
| BD-g-3 RdRp+AD-SLC2A1 | +++ | +++ | +++ | + | +++ | ++ | ++ | Y |
| BD-g-3 RdRp+AD-MAP1B | +++ | +++ | +++ | ++ | +++ | +++ | ++ | Y |
| BD-g-3 RdRp+AD-PSAP | +++ | +++ | +++ | + | +++ | +++ | +++ | Y |
| BD-g-3 RdRp+AD-BTB6 | +++ | +++ | +++ | ++ | +++ | +++ | +++ | Y |
| BD-g-3 RdRp+AD-FEZ1 | +++ | +++ | +++ | + | +++ | +++ | ++ | Y |
| BD-g-3 RdRp+AD-H2AFY2 | +++ | +++ | +++ | + | +++ | +++ | ++ | Y |
| BD-g-3 RdRp+AD-PDCL | +++ | + | ++ | + | + | - | - | N |
| BD-g-3 RdRp+AD-LSAMP | +++ | + | ++ | + | + | - | - | N |
| BD-RdRp+AD-APLP1 | +++ | + | ++ | + | + | - | - | N |
| BD-g-3 RdRp+AD-MARCKSL1 | +++ | + | ++ | + | ++ | + | - | N |
| BD-g-3 RdRp+AD-CIRBP | +++ | ++ | ++ | + | + | - | - | N |
| BD-g-3 RdRp+AD-RNF187 | +++ | ++ | ++ | + | ++ | + | - | Y |
| BD-g-3 RdRp+AD-ARM2 | +++ | +++ | ++ | + | +++ | ++ | + | Y |
| BD+AD-COG1 | +++ | - | - | - | - | - | - | |
| BD+AD-RTN1 | +++ | - | - | - | - | - | - | |
| BD+AD-EEF1A1 | +++ | - | - | - | - | - | - | |
| BD+AD-TUBB | +++ | - | - | - | - | - | - | |
| BD+AD-ZDHHC6 | +++ | - | - | - | - | - | - | |
| BD+AD-MACF1 | +++ | - | - | - | - | - | - | |
| BD+AD-TMEM66 | +++ | - | - | - | - | - | - | |
| BD+AD-ACTG1 | +++ | - | - | - | - | - | - | |
| BD+AD-SLC2A1 | +++ | - | - | - | - | - | - | |
| BD+AD-MAP1B | +++ | ++ | ++ | ++ | +++ | ++ | + | |
| BD+AD-PSAP | +++ | - | - | - | - | - | - | |
| BD+AD- BTB6 | +++ | - | - | - | - | - | - | |
| BD+AD-FEZ1 | +++ | - | - | - | - | - | - | |
| BD+AD-H2AFY2 | +++ | - | - | - | - | - | - | |
| BD+AD-PDCL | +++ | - | - | - | - | - | - | |
| BD+AD-LSAMP | +++ | - | - | + | - | - | - | |
| BD+AD-APLP1 | +++ | - | - | + | - | - | - | |
| BD+AD-MARCKSL1 | +++ | - | - | + | - | - | - | |
| BD+AD-CIRBP | +++ | + | - | + | - | - | - | |
| BD+AD-RNF187 | +++ | - | - | - | - | - | - | |
| BD+AD-ARM2 | +++ | - | - | + | - | - | - | |
Y2H gold strain was transformed in indicated combinations and plated on media lacking Leucine, Tryptophan (LT-). Eight random colonies from each transformants were replica plated to media containing various selection markers, as indicated and their growth monitored over a period of four days. Abbreviations are as in Table 1. Y: Positive, N: Negative. PPI status: Protein-protein interaction status. Data represents the summary of 3 independent experiments.