Fig 9. Topical vitamin D treatment does not affect wound closure rate in a human organ culture wounding model but wounded corneas express a higher level of the vitamin D activating enzyme, CYP27B1.

(A, B) Corneal wounds were monitored by fluorescein staining every 6 hours for 24 hours. Data were analyzed with repeated measures ANOVA and Bonferroni’s correction for multiple comparisons. (n = 5) (C) Corneal epithelium was collected at the time of wounding (initial) and after 48 hours of regrowth (regrown) and CYP27B1 expression was determined by RT-PCR. Data were analyzed by Student’s two-tailed t-test. * = p<0.05 (n = 4).