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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Bone Miner Res. 2015 Jan;30(1):64–70. doi: 10.1002/jbmr.2313

Figure 4. Characterization of Bmp2, Bmp4, and Noggin transcription in cultured skeletal lineage cells and their precursors.

Figure 4

(A and B) Epiphyseal chondrocytes or BMSCs (osteoblasts) were isolated from the femurs of 3–4 week-old 5′ Bmp2 BAC, 5′ Bmp4 BAC, or Nog-LacZ mice, cultured with ascorbate and inorganic phosphate for up to 3 weeks, and stained with X-gal. (A) Time course showing a week-by-week progression of staining, beginning at the point when mineralization-supporting media was first added to the cultures (Day 0). (B) Day 21 cultures counter-stained with Alizarin Red to highlight mineralized nodules (shown at low magnification in insets).