Table 1. Compositional analysis of OMVs from P. gingivalis, T. denticola and T. forsythia.

Parametera	P. gingivalis	T. denticola	T. forsythia
OMVs/mL cultureb	4.98 ± 0.77 x 1012	5.54 ± 1.47 x 1011	2.00 ± 0.65 x 1012
size rangec	37–315 nm (121 nm)	18–247 nm (75 nm)	59–397 nm (158 nm)
OMVs/106 nm2 cell surface aread	936 ± 137	158 ± 37	437 ± 90
Protein Contente ng/108 OMVs	38.8 ± 4.59	17.3 ± 2.47	45.4 ± 20.5
LPS/LOS Contentf ng/108 OMVs	17.5 ± 5.21	0.11 ± 0.04	12.9 ± 0.64
Lipo-protein/peptide Contentg ng/108 OMVs	16.2 ± 3.39	7.35 ± 0.39	14.8 ± 0.23
Peptidoglycan Contenth ng/108 OMVs	2.97 ± 0.48	1.60 ± 0.35	5.21 ± 1.77
DNA Contenti pg/108 OMVs	11.7 ± 3.28	7.44 ± 5.86	32.6 ± 6.74
RNA Contentj pg/108 OMVs	5.11 ± 1.00	1.12 ± 0.50	4.42 ± 1.78

^a All data are presented as mean ± standard deviation of 5 biological replicates.

^b OMVs labelled with green fluorescent dye PHK-67 were counted using an Apogee A50-Micro Flow Cytometer in samples taken from late exponential cultures of each bacterium.

^c Size range of OMVs was determined using Dynamic Light Scattering on a Malvern high performance particle sizer. Modal diameter in parenthesis.

^d Total cell surface area was calculated using whole bacterial cell counts per mL of culture determined with a Cell Lab Quanta SC Flow Cytometer and the cellular dimensions for each species [34]

^e Protein concentration determined by Qubit assay.

^f Biologically active LPS was measured using HEK-Blue TLR4 Cells and a standard curve of E. coli LPS.

^g Biologically active lipoproteins were measured using HEK-Blue TLR2 Cells and a standard curve of Pam3CSK4.

^h Peptidoglycan was determined using a Wako SLP reagent set.

ⁱ DNA was measured using a Qubit dsDNA HS assay kit.

^j RNA was measured using a Qubit RNA assay kit.