Table 2. Microsatellites loci and primers.
Locus | Microsatellitea | Allele size range (bp) | Number of alleles | Primers U/Lb |
---|---|---|---|---|
NG25 | (GT)8 | 110–116 | 4 | AATATTGCTGATGCGAAGGG/AATTGCACCACCAACTCCG |
NG42-1 | (CT)5…(ACC)7 | 220–226 | 4 | GCAGCACTCACTCCTCCTC/CACCTCTCCCTCTTACAACAG |
NG42-2 | (GGT)7…(GGT)4 | 222–252 | 5 | GATTTCCTGCTGGACGATGA/CCCAAGTCGTCCATGATGAGA |
NG69 | (TTC)7…(CAT)6G(GGT)6…(CTT)6 | 238–245 | 3 | CAACCTGTTCCCAAGATTTGTAAG/TATGGATAAGGAAAGAAGTGATACAAG |
NG141-3 | (TG)6…(TG)5…(TG)4…(GT)6…(GT)6 | 162–216 | 10 | CTCAATGTAGAAAATGCTAAT/TGGAATGAATGGAATATACTC |
NG141-5 | (GT)11 | 153–163 | 5 | GAGAGTATATTCCATTCATTCCA /TTGATCATCCTCATCATTCCACC |
a repetitions of microsatellite are indicated for KUL strain.
b U: Upper primer (5'-3') and L: lower primer 5'-3'.