Fig 5. Phox KO macrophages present reduced parasitism.

Inflammatory macrophages obtained from peritoneal cavity of C57BL/6 WT and Phox KO mice were subjected to infection with Y (A, B) and CL Brenner (C, D) strains of T. cruzi. Cells were washed to remove extracellular parasites and either fixed or re-incubated with medium for different times. The slides were stained and counted to determine the number of parasites per macrophage. A minimum of 200 macrophages were counted per group in triplicate. (A, C). The number of parasites released in the supernatant of cells between the third and seventh day after infection was quantified (B, D). Data shown are representative one of three independent experiments performed in triplicate. All data are presents as the means ± standard deviation. * indicates significant differences between marked bars or points, p<0.05, two-way ANOVA test with Bonferroni post-test.