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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Exp Cell Res. 2016 Feb 24;342(1):32–38. doi: 10.1016/j.yexcr.2016.02.015

Figure 4. C646 induces the presence of the apoptosis protein, cleaved Caspase-3.

Figure 4

Subconfluent primary cultures of MEMM cells were treated with 0–15 μM C646 for 24 hours and then total cell lysates analyzed by Western blotting for active (cleaved) Caspase-3 (top panel), stripped, and then reprobed with anti-β-tubulin antibodies (bottom panel). There was no detectable cleaved Caspase-3 in control samples or in cultures treated with 10 μM C646. Low levels of cleaved Caspase-3 were seen following treatment of cells with 12.5 μM C626. Increased expression of cleaved Caspase-3 was seen following treatment of cells with 15 μM C646.