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. Author manuscript; available in PMC: 2017 May 5.
Published in final edited form as: Mol Cell Endocrinol. 2016 Feb 9;426:11–21. doi: 10.1016/j.mce.2016.02.007

Fig. 1. E2 treatment increases PR expression in the mammary gland.

Fig. 1

Ovariectomized female mice were treated with oil or E2 for 7 days. ERα and PR expression was examined in mammary gland sections of 15 week-old virgin mice, 15 week-old primiparous mice, and 9 month-old retired breeders using immunohistochemistry and immunofluorescent microscopy. Methyl green or DAPI counter staining was included to identify cell nuclei for immunohistochemistry and immunofluorescence, respectively. Scale bars indicate 25 µm. Images from 6 mice were collected in each group. Representative images are shown.