Figure 4.

The involvement of NHE1 activity in the PPARα antiapoptotic effect. NRK-52E cells were transfected with pcPPAR or blank vectors, pretreated with or without 1 μmol/L cariporide for 30 min and then treated with or without 3 mmol/L gentamicin for 24 h. (A) Representative flow cytometric data of gentamicin-induced apoptosis in the pcPPAR-transfected cells. Treated cells were stained with annexin V/PI and analyzed by flow cytometry. The sum of cell percentages in quadrants 2 and 4 represents the percentage of apoptosis. P, pcPPAR transfection; G, gentamicin treatment; Cari, cariporide treatment. (B) The gentamicin-induced apoptotic percentage in the pcPPAR-transfected cells. Results are means ± SD (n = 3). *P < 0.05 by Student t test. (C) The influence of cariporide on gentamicin-induced apoptotic signals in the pcPPAR-transfected NRK-52E cells. Total proteins of NRK-52E cells were analyzed by Western blotting with the specific antibodies as indicated. GAPDH was used as a loading control. Relative increases in the protein bands are also presented in a bar chart form. Results are means ± SD (n = 3). *P < 0.05 versus the blank vector group without any treatment. ^#P < 0.05 versus the blank vector group with gentamicin treatment. **P < 0.05 versus the pcPPAR group with gentamicin treatment. Statistical differences between the two groups were determined using a Student t test.