Table 2.
Comparison of murine and human fresh versus cryopreserved M(IL-4) by real-time PCR.
| Fold-change over M(−) | |||
|---|---|---|---|
|
|
|||
| Species | Gene | Fresh M(IL-4) | Cryopreserved M(IL-4) |
| Mouse | Arg1 | 183,966 ± 54,719a,b | 1,254 ± 552 |
| Nos2 | 11.06 ± 3.05a,b | 2.31 ± 0.825 | |
| Chi3l3 (Ym1) | 2,296 ± 678a,b | 177 ± 17 | |
| Retnla (Relm-α) | 1,955,323 ± 470,371a,b | 72,331 ± 20,697 | |
| Human | CD14 | 0.110 ± 0.0367a,b | 0.0231 ± 0.0135a |
| MRC1 | 10.66 ± 1.64a,b | 5.49 ± 1.41a | |
| CCL18 | 64.75 ± 27.91a,b | 4.27 ± 1.72 | |
Mouse bone marrow–derived macrophages were treated with IL-4 for 48 h and compared before and after cryopreservation for AAM marker expression (Arg1, Chi3l3, Retnla) and Nos2 as a negative control. Likewise, fresh and frozen human monocyte–derived M(IL-4)s from healthy blood donors were compared on their capacity to downregulate CD14 and upregulate MRC1 and CCL18 transcripts. M(−), undifferentiated macrophage. Mouse, n = 7; human, n = 9. p < 0.05:
a
compared with M(−);
b
compared with cryopreserved M(IL-4).