Table 3.
Effect of media on the cell density of induced and uninduced culture.
| Growth medium | IPTG (μM) | Cell density at the time of induction (OD600) | Cell density after 3 h of post-induction (OD600) |
|---|---|---|---|
| Nutrient broth | 0 | 0.56 | 1.39 |
| Nutrient broth | 100 | 0.55 | 1.14 |
| LB broth | 0 | 0.6 | 2.27 |
| LB broth | 100 | 0.59 | 1.52 |
| 2× LB broth | 0 | 0.63 | 1.92 |
| 2× LB broth | 100 | 0.64 | 1.53 |
| Terrific broth | 0 | 0.68 | 3.13 |
| Terrific broth | 100 | 0.67 | 0.92 |