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. 2016 Mar 31;6:22. doi: 10.1186/s13578-016-0088-4

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Duplex indel mutations in single embryo. a, d T7E1 assay of duplex indel mutations in the loci of pax3 and zic1 (a) or snail1 and snail2 (d) in five single embryos. b, e Quantification of the T7E1 assay in (a) or (d). c, f Representative sequencing data confirmed the indel mutations induced at the two target loci in one single embryo