Table 1.

His-Tol2 and Tol2 mRNA as sources of transposase in vivo

Somatic F0 pattern	Tol2 form	Germline trapping and expression frequency	Average F0 germline mosaicism
Cat II.	mRNA	65 % (13/20)	27 %
Cat III.	mRNA	88 % (35/40)	39 %
Cat III.	Protein	76 % (13/17)	39 %
Cat IV.	Protein	90 % (9/10)	51 %

Analyses of somatic and germline transmission of reporter genes are shown. Fish injected with either mRNA or His-Tol2 were characterized by different F0 somatic patterns and assessed for corresponding germline trapping frequency, estimated as the percentage of F0 fish producing GFP-positive offspring. The number of fish screened is shown in parentheses. The F0 germline mosaicism rate was determined by the percentage of GFP-positive F1 offspring from a founder outcross. The average mosaicism rate for founder fish is listed from each different category