Table 2.
Site | Sequence | Chr. | Refseq |
---|---|---|---|
1 | aaatatttaccaagcaac-5′-Tol2-3′-caagcaacacgttcagtg | 8 | Intron of gfra2 |
2 | ataatttcctcttatttg-5′-Tol2-3′-cttatttgcatgtcagat | 13 | Intergenic |
3 | cgcatgctaacttataga-5′-Tol2-3′-cttatagaggaggtgccc | 8 | Exon of wu: fb79a07 |
4 | aaacgttcctcctaacac-5′-Tol2-3′-cctaacacagttagatgg | 3 | Intergenic |
5 | caacacatgactcgttgg-5′-Tol2-3′-ctcgttggccatatgcta | 15 | Intergenic |
6 | gggaatatgtgttattaa-5′-Tol2-3′-gttattaactgcgtccca | 4 | Repetitive sequences |
7 | agctgtctcttctgtgtc-5′-Tol2-3′-tctgtgtcattcagtctc | 3 | Intron of LOC557901 |
8 | tgtcagagatctaggtca-5′-Tol2-3′-ctaggtcagatggaggaa | 25 | Intergenic |
Tol2 insertion sites generated by His-Tol2 protein co-injection were cloned from F1 transgenic fish. Examples of 8-bp genomic sequence duplication (in bold) were shown with insertion loci information