Table 2.

Transposase-mediated germline integration

Site	Sequence	Chr.	Refseq
1	aaatatttaccaagcaac-5′-Tol2-3′-caagcaacacgttcagtg	8	Intron of gfra2
2	ataatttcctcttatttg-5′-Tol2-3′-cttatttgcatgtcagat	13	Intergenic
3	cgcatgctaacttataga-5′-Tol2-3′-cttatagaggaggtgccc	8	Exon of wu: fb79a07
4	aaacgttcctcctaacac-5′-Tol2-3′-cctaacacagttagatgg	3	Intergenic
5	caacacatgactcgttgg-5′-Tol2-3′-ctcgttggccatatgcta	15	Intergenic
6	gggaatatgtgttattaa-5′-Tol2-3′-gttattaactgcgtccca	4	Repetitive sequences
7	agctgtctcttctgtgtc-5′-Tol2-3′-tctgtgtcattcagtctc	3	Intron of LOC557901
8	tgtcagagatctaggtca-5′-Tol2-3′-ctaggtcagatggaggaa	25	Intergenic

Tol2 insertion sites generated by His-Tol2 protein co-injection were cloned from F1 transgenic fish. Examples of 8-bp genomic sequence duplication (in bold) were shown with insertion loci information