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. 2016 Apr 1;6:11. doi: 10.1186/s13601-016-0100-4

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© Santos et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Gating with CD123/HLA-DR led to the loss-to-analysis of basophils and underestimation of basophil activation. In a representative experiment, basophils were gated as SSClow/CD123^bright/HLA-DR− (a) or SSC^low/CD203c+/CD123+/HLA-DR− (b). The percentage of CD63+ basophils is represented in different conditions: negative control, peanut extract 100 ng/ml and peanut-dose–response curve. Note the bi-exponential display [38]