Table 3.
Diagnostic accuracy of the basophil activation test to peanut using different gating strategies
| Gating strategy | ||
|---|---|---|
| CD123+ HLADR− | CD203c+ CD123+ HLADR− | |
| Optimal cut-off | 6.8 | 8.1 |
| AUC ROC for the cut-off | 0.91 | 0.97 |
| Accuracy (%) | 91 | 97 |
| Sensitivity (%) | 88 | 98 |
| Specificity (%) | 94 | 96 |
| PPV (%) | 93 | 95 |
| NPV (%) | 90 | 98 |
| %True positives | 40 | 45 |
| %False positives | 3 | 2 |
| %True negatives | 51 | 52 |
| %False negatives | 5 | 1 |
| LR+ | 14.7 | 24.4 |
| LR− | 0.13 | 0.02 |
The optimal cut-off was determined for the %CD63+ basophils following stimulation with 100 ng/ml of peanut extract
PPV positive predictive value, NPV negative predictive value, LR+ positive likelihood ratio, LR− negative likelihood ratio