Fig. 4.

Retention of intron 20 of the GON4L gene in a Fleckvieh calf with dwarfism. a RT-PCR products were separated by 2 % agarose gel electrophoresis. Lanes 1, 2, and 3 show 348 bp RT-PCR products from lung, lymph nodes, and liver of a wild type animal. Lanes 4, 5, and 6 show 348 and 1488 bp RT-PCR products from lung, lymph nodes, and liver of a homozygous calf. M size marker, N negative RT-PCR control. b Schematic representation of exons 19–22 of wild type GON4L. c Schematic representation of exons 19–22 of the mutant GON4L. Blue arrows represent forward (1F) and reverse primers (1R, 2R). The red ‘X’ displays the premature translation termination at amino acid position 1492 resulting from the retention of intron 20. The light grey colour (c) displays intron 20