Fig. 1.

Active β-catenin, Prickle1 and Vangl2 are differently expressed in neuroblastoma cell lines. a Protein expression of active, de-phoshorylated β-catenin, the canonical Wnt/β-catenin target gene Axin2 and b. total β-catenin in neuroblastoma cell lines. SK-N-BE (2), SK-N-DZ, IMR-32 and Kelly are MYCN amplified neuroblastoma cells with high MycN expression. SH-SY5Y, SK-N-SH and SK-N-FI are non-MYCN amplified neuroblastoma cells expressing relatively low levels of MycN whereas, SK-N-AS and SHEP-1 do not show any MycN expression [33] c. Quantified protein expression (adjusted to β-actin) of Prickle1 and Vangl2 in neuroblastoma cell lines. Proteins were determined with western blotting. d mRNA expression in neuroblastoma cell lines SK-N-BE (2), SK-N-DZ, SK-N-AS, SH-EP1 and SH-SY5Y, assessed by quantitative real-time PCR, the data displayed is the mean ± S.D. of three determinations