Table 2.
Nonsynonymous nucleotide mutations identified between the daptomycin non-susceptible Enterococcus faecium (EFM02) strain and the daptomycin-susceptible Enterococcus faecium (EFM01) strain
| Genes | RecJ | MutL | FusA | – | HyuA | DapB | – | ManX | YcaB | EbpR |
|---|---|---|---|---|---|---|---|---|---|---|
| locus_tag | EFAU085_01331 | EFAU085_00136 | EFAU085_00055 | EFAU085_00149 | EFAU085_00226 | EFAU085_00409 | EFAU085_00744 | EFAU085_00813 | EFAU085_01095 | EFAU085_01417 |
| Predicted gene products | DNA repair protein | DNA mismatch repair protein | Translation elongation factor G | ABC transporter | Hydantoinase/oxoprolinase | Dihydrodipicolinate reductase | Membrane protein | PTS system, Mannose/fructose/sorbose-specific IIAB component | Calcium-translocating P-type ATPase, PMCA-type | M protein trans-acting positive regulator |
| Predicted amino acid change | Tyr434 Cys | Leu286 | Arg626 Cys | Gly190 Asp | Thr570 Ala | Ala190 Val | Phe89 fs | Met1 | Ala108 fs | Pro199 Leu |
| Nucleotide mutation | 1301A > G | 1876C > T | 857T > A | 569G > A | 1708A > G | 569C > T | 260delT | 1A > G | 315delA | 596C > T |
| Previous reports on the same predicted proteins associated with DNSE | [2] | [2, 5, 21] |
| Genes | GlpQ | – | – | – | PspC | – | AmpC | ytpA | – |
|---|---|---|---|---|---|---|---|---|---|
| locus_tag | EFAU085_01796 | EFAU085_01902 | EFAU085_01910 | EFAU085_0195 | EFAU085_02050 | EFAU085_02219 | EFAU085_02548 | EFAU085_02618 | EFAU085_02818 |
| Predicted gene products | Glycerophosphodiester phosphodiesterase family protein | Hypothetical protein | Hypothetical protein | HD domain protein | PspC domain protein | Hypothetical protein | Beta-lactamase | Alpha/beta hydrolase family protein | Hypothetical protein |
| Predicted amino acid change | Ile283Phe | Trp176 | Asn125 fs | Trp118 Arg |
Lys5 fs | Ser23 fs | Asn265 fs | Glu59Gly | Arg220 fs |
| Nucleotide mutation | 847A > T | 527G > A | 374delA | 352T > C | 14delA | 68delC | 794delA | 176A > G | 658delA |
| Previous reports on the same predicted proteins associated with DNSE | [ 5], [21] | [ 2], [5] |
Reference strain: Enterococcus faecium Aus0085 [8]
ABC ATP-binding cassette, A adenine, Ala alanine, Arg arginine, Asn asparagine, Asp aspartic acid, C cytosine, Cys cysteine, del deletion, DNSE daptomycin non-susceptible Enterococcus, fs frameshift, G guanine, Glu glutamic acid, Gly glycine, Ile isoleucine, Leu leucine, Lys lysine, Met methionine, Phe phenylalanine, PMCA plasma membrance Ca2 + ATPase, Pro proline, PTS Phosphotransferase sytem, psPC phage shock protein C, Ser serine, Tyr tyrosine, T thymine, Thr threonine, Trp tryptophan, Val valine