Fig. 4.

STED imaging of γ-secretase in synapses with quadruple-staining. (a) γ-Secretase (red), postsynaptic PSD95 (green) and presynaptic synaptobrevin (blue). Neuronal structure is visualized by f-actin in an overlaid non-STED confocal channel (white). (b) A zoomed axon and dendrite from (a) indicating an active dendritic spine with the presynapse and postsynapse, indicated by synaptobrevin in blue (upper arrow) and PSD95 in green (lower arrow) respectively. (c) Merged image of (b) without Phalloidin indicating that γ-secretase (red) is mainly found in the postsynapse (lower arrow), co-localized with PSD95 (green). Small amounts of γ-secretase (red) is found in the presynapse (upper arrow), co-localized with synaptobrevin (blue). Individual channels of (d) Postsynaptic marker PSD95, (e) γ-Secretase, (f) Synaptobrevin and (e) Confocal image of f-actin